Journal: bioRxiv

Article Title: Unexpected mechanisms of sex-specific memory vulnerabilities to acute traumatic stress

doi: 10.1101/2025.03.25.645300

Figure Lengend Snippet: ( A ) Bivalent genes possess both H3K4me3 and H3K27me3 histone posttranslational modifications. These genes are thus poised for transcriptional activation or repression. Upon an appropriate stimulus, loss of H3K27me3 promotes a permissive state (H3K4me3 only), while loss of H3K4me3 reinforces a repressive state (H3K27me3 only). Chromatin state was analyzed in naïve (stress-free) male, proestrous female, and estrous female mouse hippocampus. ( B ) Summary counts of unique gene promoter regions (+/-100bp from TSS) enriched in permissive or repressive chromatin in naïve ATS-vulnerable or resilient mice. ATS-resilient females show higher bivalent and repressive states while males and proestrous females show higher permissive states. ( C ) Permissive state promoters (H3K4me3 only) were more numerous in male and proestrous females than estrous females. ( D ) In parallel, more repressive state promoters (H3K27me3 only) were identified in estrous females than males and proestrous females. ( E ) Promoter bivalency (both K3K4me3 and K3K27me3) was more frequent in estrous females. ( F ) Top 15 enriched motifs including ER-associated Egr1 and TFAP2 family of transcription factors were bivalent in estrous females and ( G ) permissive in proestrous females and males. ( H ) Summary motif figure showing that transcription factor motifs that are enriched in bivalent chromatin unique to estrous females are instead permissive in proestrous females and males (Motif-containing genes differ by groups).

Article Snippet: The nuclei pellets were equally divided for incubation with either H3K27me3 antibodies (Active Motif, 39055) or H3K4me3 antibodies (Abcam, Ab8580).

Techniques: Activation Assay

Journal: bioRxiv

Article Title: Unexpected mechanisms of sex-specific memory vulnerabilities to acute traumatic stress

doi: 10.1101/2025.03.25.645300

Figure Lengend Snippet: ( A ) Gene expression levels across different promoter chromatin states. Promoters (±100 bp from TSS) were categorized as H3K4me3-only, H3K27me3-only, or bivalent (H3K4me3 + H3K27me3) and intersected with RNA-seq expression data. Violin plots show that genes marked by H3K4me3-only (N = 11,057) have significantly higher TPM compared to bivalent (N = 3,999) and H3K27me3-only (N = 1,328) promoters (Wilcoxon test, adjusted p < 0.05 for all comparisons). ( B ) Principal component plot for RNA-seq data of male, proestrous, or estrous female mouse hippocampus. The three biological replicates per sex and estrous cycle phase (N = 3/group) show consistent clustering. Males and proestrous females cluster closer together on PC1. ( C ) Upset plot portraying unique and overlapping differential expressed genes in all comparisons. ( D ) Gene ontology enrichment analysis shows top 10 enriched GO terms, with some relating to synaptic signaling pathways in the estrous vs. proestrous female comparison ( E ) whereas top 10 enriched GO terms for the estrous female vs. male comparison are metabolic-related terms. ( F ) Overlap analysis of DEGs in male, proestrous female, and estrous female hippocampus and ERα targets (targets identified using dataset described in shows significant overlap by Fisher’s exact test of the estrous vs. proestrous and the estrous vs. male DEGs. ( G ) A cartoon depicting the proposed mechanisms by which ATS provokes memory disturbances, governed by sex and hippocampal estrogen levels, and mediated by sex-specific estrogen receptors (ERs). Proestrous female mice were more sensitive to ATS-induced memory deficits and these deficits were more persistent than those in males.

Article Snippet: The nuclei pellets were equally divided for incubation with either H3K27me3 antibodies (Active Motif, 39055) or H3K4me3 antibodies (Abcam, Ab8580).

Techniques: Gene Expression, RNA Sequencing, Expressing, Protein-Protein interactions, Comparison

Journal: iScience

Article Title: Amniotic epithelial Cell microvesicles uptake inhibits PBMCs and Jurkat cells activation by inducing mitochondria-dependent apoptosis

doi: 10.1016/j.isci.2025.111830

Figure Lengend Snippet:

Article Snippet: Total protein was extracted from each sample using RIPA lysis buffer (#R0278, Sigma-Aldrich, St. Louis, MO, USA) supplemented with Phosphatase Inhibitor (#39055; SERVA Electrophoresis GmbH, Heidelberg, Germany) and Protease Inhibitor Cocktails (#P2714; Sigma-Aldrich, St. Louis, MO, USA) diluted according to manufacturer’s instruction.

Techniques: Recombinant, Electrophoresis, Protease Inhibitor, Blocking Assay, Luciferase, Proliferation Assay, Flow Cytometry, Transgenic Assay, Software, Control

Journal: iScience

Article Title: Amniotic epithelial Cell microvesicles uptake inhibits PBMCs and Jurkat cells activation by inducing mitochondria-dependent apoptosis

doi: 10.1016/j.isci.2025.111830

Figure Lengend Snippet:

Article Snippet: Phosphatase Inhibitor , SERVA Electrophoresis GmbH , Cat# 39055.

Techniques: Recombinant, Electrophoresis, Protease Inhibitor, Blocking Assay, Luciferase, Proliferation Assay, Flow Cytometry, Transgenic Assay, Software, Control